What is Magnetic Beads DNA Extraction Kit for Tissue?
Our Magnetic Beads DNA Extraction Kit for Tissue is a specialized scientific tool designed to isolate high-quality DNA from various tissue samples. Using magnetic bead technology, this kit efficiently captures DNA molecules from tissues like biopsies, organs, or preserved samples, making it essential for genetic research and diagnostics. The process is streamlined and user-friendly, reducing the risk of contamination while ensuring reliable results.
This kit is particularly valuable for handling complex tissue matrices, which often contain proteins, lipids, and other cellular debris that can interfere with DNA extraction. By leveraging magnetic properties, the kit simplifies the separation of DNA from these impurities, offering a clean and concentrated DNA output suitable for downstream applications such as PCR, sequencing, or cloning.
Technical Specifications
| Model | Method | Sample | Format | Workflow |
|---|---|---|---|---|
| M-TIS-20R | Magnetic Beads | Tissue | 20R | Manual |
| M-TIS-50R | Magnetic Beads | Tissue | 50R | Manual |
| M-TIS-100R | Magnetic Beads | Tissue | 100R | Manual |
| M-TIS-200R | Magnetic Beads | Tissue | 200R | Manual |
| M-TIS-8R | Magnetic Beads | Tissue | 8R | Semi-automated or fully automated extraction equipment |
| M-TIS-24R | Magnetic Beads | Tissue | 24R | Semi-automated or fully automated extraction equipment |
| M-TIS-48R | Magnetic Beads | Tissue | 48R | Semi-automated or fully automated extraction equipment |
| M-TIS-72R | Magnetic Beads | Tissue | 72R | Semi-automated or fully automated extraction equipment |
| M-TIS-96R | Magnetic Beads | Tissue | 96R | Semi-automated or fully automated extraction equipment |
Working Principle
The principle behind the Magnetic Beads DNA Extraction Kit for Tissue involves the use of magnetic beads coated with a silica-based surface that binds DNA molecules under specific buffer conditions. When tissue samples are lysed, the DNA is released and binds to these beads. A magnetic field is then applied to separate the bead-DNA complexes from other cellular components.
After washing away impurities, the DNA is eluted from the beads in a pure form. This method leverages the high affinity of silica for DNA in the presence of chaotropic salts, ensuring efficient capture and minimal loss, making it a robust and scalable approach for DNA isolation.
Performance
The performance of our Magnetic Beads DNA Extraction Kit for Tissue is characterized by high efficiency and consistency. It achieves excellent DNA yield and purity, with typical results showing A260/A280 ratios between 1.8 and 2.0, indicating minimal protein or RNA contamination. This ensures that the extracted DNA is of optimal quality for sensitive molecular techniques.
In rigorous testing, the kit has demonstrated rapid processing times, often completing extractions in under an hour, and robust performance across a wide range of tissue types, including fresh, frozen, and formalin-fixed paraffin-embedded (FFPE) tissues. Its reliability makes it a preferred choice for laboratories requiring reproducible outcomes in high-throughput environments.
Application
This kit is widely used in various applications, such as genetic research, medical diagnostics, and forensic analysis. In research settings, it facilitates studies on gene expression, mutation detection, and genome sequencing from tissue samples. Clinically, it supports diagnostic tests for diseases like cancer, where DNA extraction from tumor tissues is crucial for identifying genetic mutations.
Additionally, the kit is employed in forensic science for DNA profiling from tissue evidence and in agricultural or environmental studies for analyzing plant or animal tissues. Its versatility across different fields underscores its importance in advancing scientific knowledge and practical solutions.
Core Features and Advantages
Core features of this kit include its high specificity for DNA binding, ease of use with minimal hands-on time, and compatibility with automation for high-throughput processing. The magnetic bead technology eliminates the need for centrifugation or filtration, reducing physical handling and the risk of sample cross-contamination.
Advantages encompass superior DNA purity and yield, adaptability to various tissue types, and cost-effectiveness due to reusable magnetic stands. These benefits make it an ideal solution for laboratories seeking efficient, reliable, and scalable DNA extraction methods.