Magnetic Beads DNA Extraction Kit for Blood
Magnetic Beads DNA Extraction Kit for Tissue
Magnetic Beads DNA Extraction Kit for Plant
Magnetic Beads DNA Extraction Kit for Cell
Magnetic Beads DNA Extraction Kit for Saliva
Magnetic Beads DNA Extraction Kit for Microorganisms
Magnetic Beads DNA Extraction Kit for Forensic Samples
Magnetic Beads DNA Extraction Kit for Environmental Samples
Magnetic Beads DNA Extraction Kit for FFPE Samples
What is Magnetic Beads DNA Extraction Kit?
The Magnetic Beads DNA Extraction Kit is a laboratory-based kit that uses magnetic bead separation technology to efficiently purify DNA.
This kit utilizes tiny, superparamagnetic silica-based beads coated with a special surface, which specifically adsorb nucleic acids in high-salt environments, while impurities such as proteins remain in the supernatant. Under the influence of an external magnetic field, the magnetic bead-DNA complex is rapidly captured and immobilized. The wastewater is then discarded to complete the cleaning process, thoroughly removing any inhibitory components. Finally, a low-salt eluent is used to separate the pure DNA from the beads, yielding high-quality DNA. Magnetic bead kits easily integrate with a variety of commercially available semi-automated and fully automated workstations, rapidly processing large volumes of samples while eliminating contamination and human error. They offer flexible application, capable of processing ultra-micro, trace, and standard sample types simultaneously according to the automation program's various settings. They have become a mainstream method in high-end scientific research, clinical diagnostics, and large-scale genetic testing.
Technical Specifications
| Model | Method | Sample | Format | Workflow |
|---|---|---|---|---|
| M-BLD | Magnetic Beads | Blood / Blood Stains | 20R, 50R, 100R, 200R / 8R, 24R, 48R, 72R, 96R | Manual / Automation |
| M-CELL | Magnetic Beads | Cultured Cells | 20R, 50R, 100R, 200R / 8R, 24R, 48R, 72R, 96R | Manual / Automation |
| M-UNI | Magnetic Beads | Universal | 20R, 50R, 100R, 200R / 8R, 24R, 48R, 72R, 96R | Manual / Automation |
| M-FFPE | Magnetic Beads | FFPE Tissue | 20R, 50R, 100R, 200R / 8R, 24R, 48R, 72R, 96R | Manual / Automation |
| M-UNI | Magnetic Beads | Universal | 20R, 50R, 100R, 200R / 8R, 24R, 48R, 72R, 96R | Manual / Automation |
| M-VIR | Magnetic Beads | Virus | 20R, 50R, 100R, 200R / 8R, 24R, 48R, 72R, 96R | Manual / Automation |
| M-PLN | Magnetic Beads | Plant | 20R, 50R, 100R, 200R / 8R, 24R, 48R, 72R, 96R | Manual / Automation |
| M-SAL | Magnetic Beads | Saliva | 20R, 50R, 100R, 200R / 8R, 24R, 48R, 72R, 96R | Manual / Automation |
| M-TIS | Magnetic Beads | Tissue | 20R, 50R, 100R, 200R / 8R, 24R, 48R, 72R, 96R | Manual / Automation |
Working Principle
The principle behind the Magnetic Beads DNA Extraction Kit involves the use of silica-coated magnetic beads that bind to DNA under specific buffer conditions, typically involving chaotropic salts that denature proteins and facilitate DNA attachment. When a magnetic field is applied, the beads with bound DNA are pulled to the side of the container, allowing the supernatant containing impurities to be discarded.
Subsequent washing steps with ethanol-based solutions remove remaining contaminants, and finally, the DNA is eluted in a low-salt buffer or water, releasing pure DNA ready for use. This magnetic separation principle minimizes physical handling and reduces the risk of cross-contamination, making it a robust and efficient method.
Performance
The performance of a Magnetic Beads DNA Extraction Kit is characterized by its high yield and purity of extracted DNA, often achieving over 95% recovery rates with minimal degradation. This ensures that the DNA is intact and suitable for sensitive molecular techniques. The kit is optimized for consistency, providing reproducible results across multiple samples, which is crucial for experiments requiring reliable data.
Additionally, it demonstrates excellent specificity by effectively binding to DNA while excluding impurities, leading to cleaner extracts. Performance metrics include fast processing times, typically completing extraction in under an hour, and compatibility with automated systems for high-throughput applications, enhancing laboratory efficiency.
Application
Magnetic Beads DNA Extraction Kits are widely used in various fields, including clinical diagnostics for genetic testing and mutation analysis, where pure DNA is essential for accurate results. In research settings, they are employed for studying genomes from samples like blood, tissues, or environmental sources, enabling advancements in genomics and biotechnology.
Forensic laboratories utilize these kits to extract DNA from challenging samples such as hair or saliva for identification purposes. They are also applied in agricultural and food safety testing to detect pathogens or verify authenticity, showcasing their versatility across diverse scientific disciplines.
Core Features and Advantages
Core features of the Magnetic Beads DNA Extraction Kit include its high binding capacity, allowing for efficient DNA capture from small sample volumes, and its scalability from manual to automated workflows. The kit is designed to be rapid, often completing extractions in less time compared to column-based methods, and it requires minimal equipment, just a magnet stand or automated magnetic separator.
Advantages encompass improved DNA purity by reducing inhibitor carryover, which is critical for downstream applications like PCR. It is also cost-effective due to reusable magnetic beads in some formats and offers flexibility for various sample types, from blood to environmental samples, enhancing its utility in diverse laboratory settings.