What is Silica Beads DNA Extraction Kit for Tissue?
A Silica Beads DNA Extraction Kit for Tissue is a specialized laboratory tool designed to isolate DNA from tissue samples using silica bead technology. This kit is engineered to handle the unique challenges of tissue samples, which may contain complex cellular structures, connective materials, or preservatives that can interfere with DNA purity. The process involves binding DNA molecules to silica beads under specific conditions, allowing for efficient separation from other cellular components.
In molecular biology and genetics research, extracting high-quality DNA from tissues is crucial for applications such as gene expression studies, sequencing, and diagnostic testing. This kit simplifies the extraction process, making it accessible for various skill levels while ensuring reliable results. It is particularly valuable for researchers working with biopsy samples, archived tissues, or animal and plant tissues, where maintaining DNA integrity is essential for downstream analyses.
Technical Specifications
| Model | Method | Sample | Format | Workflow |
|---|---|---|---|---|
| S-TIS-20R | Silica Beads | Tissue | 20R | Manual |
| S-TIS-50R | Silica Beads | Tissue | 50R | Manual |
| S-TIS-100R | Silica Beads | Tissue | 100R | Manual |
| S-TIS-200R | Silica Beads | Tissue | 200R | Manual |
| S-TIS-8R | Silica Beads | Tissue | 8R | Semi-automated or Fully Automated |
| S-TIS-24R | Silica Beads | Tissue | 24R | Semi-automated or Fully Automated |
| S-TIS-48R | Silica Beads | Tissue | 48R | Semi-automated or Fully Automated |
| S-TIS-72R | Silica Beads | Tissue | 72R | Semi-automated or Fully Automated |
| S-TIS-96R | Silica Beads | Tissue | 96R | Semi-automated or Fully Automated |
Working Principle
The principle behind the Silica Beads DNA Extraction Kit for Tissue revolves on the affinity of DNA molecules for silica surfaces under high-salt conditions. When tissue samples are lysed using provided buffers, cellular components are broken down, releasing DNA into the solution. Silica beads are then added, and in the presence of chaotropic salts, DNA binds selectively to the beads due to hydrogen bonding and hydrophobic interactions.
Subsequent washing steps remove impurities like proteins, lipids, and salts, leaving the DNA bound to the beads. Finally, a low-salt elution buffer is used to release the pure DNA into a clean solution, ready for further analysis. This method leverages the physical properties of silica to achieve efficient separation without the need for organic solvents, making it safe and user-friendly while ensuring high DNA recovery rates.
Performance
The performance of the Silica Beads DNA Extraction Kit for Tissue is characterized by its high yield and purity of extracted DNA. It efficiently handles a wide range of tissue types, from soft tissues like liver or muscle to harder samples such as bone or plant tissues, adapting to varying sample complexities. The silica bead method ensures minimal degradation, preserving the DNA for sensitive applications like PCR or next-generation sequencing.
Users can expect consistent results with low levels of contaminants such as proteins or inhibitors, which is critical for accurate molecular analyses. The kit's design often includes optimized buffers and protocols that enhance binding efficiency, reducing processing time and increasing throughput. This makes it a reliable choice for both small-scale laboratory experiments and high-volume research settings, supporting reproducible science.
Application
This kit is widely used in diverse fields, including biomedical research, forensic science, and agricultural studies. In clinical settings, it aids in extracting DNA from human tissue samples for genetic testing, mutation analysis, or oncology research, helping to identify disease markers or hereditary conditions. For forensic applications, it can process tissue evidence from crime scenes, enabling DNA profiling for identification purposes.
In agriculture and environmental science, the kit is employed to extract DNA from plant or animal tissues for species identification, genetic diversity studies, or monitoring crop health. It also finds use in veterinary medicine and wildlife conservation, where tissue samples from animals are analyzed to track populations or diagnose diseases. Overall, its versatility makes it indispensable for any scenario requiring high-quality DNA from tissue sources.
Core Features and Advantages
Key features of this kit include its high specificity for DNA binding, which minimizes co-extraction of unwanted molecules, and its scalability, allowing it to process multiple samples simultaneously. The silica bead technology offers advantages such as rapid processing times, often completing extractions in under an hour, and compatibility with automated systems for high-throughput workflows.
Additionally, the kit is designed to be user-friendly, with clear protocols that reduce the risk of errors, making it suitable for educational settings or labs with varying expertise. Its reliability in producing high-purity DNA enhances downstream applications, reducing the need for repeat experiments and saving time and resources. Overall, these features make it a cost-effective and efficient solution for DNA extraction from tissues.