What is a Silica Beads DNA Extraction Kit for Cell?
A Silica Beads DNA Extraction Kit for Cell is a specialized laboratory tool designed to isolate DNA from cellular samples efficiently. It utilizes silica beads, which have a high affinity for DNA molecules under specific conditions, to bind and purify DNA from cell lysates. This kit is particularly useful for researchers and diagnosticians working with cell cultures, biopsies, or other cellular materials, as it ensures high purity and yield of DNA suitable for downstream applications like PCR, sequencing, or genetic analysis. The process is straightforward and minimizes contamination risks, making it a reliable choice for various scientific and medical purposes.
By employing silica-based technology, this kit simplifies DNA extraction without the need for hazardous chemicals like phenol or chloroform. It is an essential component in modern molecular biology workflows, enabling accurate and reproducible results. Whether you're studying gene expression, mutations, or cellular mechanisms, this kit provides a robust foundation for your experiments, ensuring that the extracted DNA is free from inhibitors and ready for advanced analyses.
Technical Specifications
| Model | Method | Sample | Format | Workflow |
|---|---|---|---|---|
| S-CELL-20R | Silica Beads | Cultured Cells | 20R | Manual |
| S-CELL-50R | Silica Beads | Cultured Cells | 50R | Manual |
| S-CELL-100R | Silica Beads | Cultured Cells | 100R | Manual |
| S-CELL-200R | Silica Beads | Cultured Cells | 200R | Manual |
| S-CELL-8R | Silica Beads | Cultured Cells | 8R | Semi-automated or Fully Automated |
| S-CELL-24R | Silica Beads | Cultured Cells | 24R | Semi-automated or Fully Automated |
| S-CELL-48R | Silica Beads | Cultured Cells | 48R | Semi-automated or Fully Automated |
| S-CELL-72R | Silica Beads | Cultured Cells | 72R | Semi-automated or Fully Automated |
| S-CELL-96R | Silica Beads | Cultured Cells | 96R | Semi-automated or Fully Automated |
Working Principle
The principle behind the Silica Beads DNA Extraction Kit for Cell revolves on the binding of DNA to silica surfaces under high salt conditions. When cells are lysed using provided buffers, DNA is released into the solution. Adding silica beads and adjusting the salt concentration promotes DNA adhesion to the beads through hydrogen bonding and hydrophobic interactions. Subsequent washing steps remove impurities like proteins, salts, and other cellular debris, leaving pure DNA bound to the beads.
Finally, a low-salt elution buffer is used to release the DNA from the silica beads, resulting in a clean, concentrated DNA sample ready for use. This method leverages the selective affinity of silica for nucleic acids, ensuring high specificity and minimizing co-purification of contaminants. It's a well-established technique praised for its simplicity and effectiveness, making it a cornerstone in DNA extraction protocols worldwide.
Performance
The performance of a Silica Beads DNA Extraction Kit for Cell is characterized by its high efficiency in DNA recovery and purity. Typically, it achieves over 90% DNA yield from cell samples, with minimal protein or RNA contamination. This high performance is crucial for sensitive applications such as next-generation sequencing or quantitative PCR, where even slight impurities can affect results. The kit is optimized for various cell types, including adherent and suspension cells, ensuring consistent outcomes across different experimental setups.
In bench testing, these kits demonstrate rapid processing times, often completing extraction in under an hour, which boosts laboratory throughput. They are designed to handle small to large sample volumes, making them versatile for both research and diagnostic settings. The reliability of silica bead-based extraction reduces the risk of false negatives or degraded DNA, providing confidence in the integrity of your genetic material for further studies.
Application
This kit finds widespread application in molecular biology and clinical diagnostics for extracting DNA from cell-based samples. It is commonly used in cancer research to isolate DNA from tumor cells for mutation analysis, in infectious disease studies to detect pathogens in cell cultures, and in genetic testing for hereditary conditions. Additionally, it supports pharmaceutical development by enabling drug efficacy studies on cellular models, where pure DNA is necessary for accurate genomic assessments.
Beyond research, it is employed in forensic science for analyzing DNA from cell samples found at crime scenes, such as skin cells or hair follicles. In agricultural biotechnology, it aids in plant cell DNA extraction for crop improvement projects. The kit's adaptability makes it a go-to solution for any scenario requiring high-quality DNA from cellular sources, ensuring that results are trustworthy and actionable across diverse fields.
Core Features and Advantages
The core features of this kit include its use of high-quality silica beads that maximize DNA binding capacity, user-friendly protocols that require minimal hands-on time, and compatibility with automated systems for high-throughput processing. Advantages encompass high DNA yield and purity, reduced risk of cross-contamination due to closed-tube designs in some kits, and scalability from microliter to milliliter sample volumes. It is also cost-effective, as it eliminates the need for expensive equipment or toxic reagents.
Moreover, the kit supports a broad range of cell types, from mammalian to bacterial cells, enhancing its utility in diverse applications. Its stability at room temperature simplifies storage and transportation, making it accessible for laboratories with varying resources. These features collectively ensure that users can achieve reliable, reproducible DNA extraction with ease, fostering advancements in research and diagnostics without compromising on quality or safety.