What is Spin Column DNA Extraction Kit for Tissue?
A Spin Column DNA Extraction Kit for Tissue is a specialized laboratory tool designed to isolate and purify DNA from tissue samples. This kit uses a spin column method, where the tissue is first broken down to release DNA, which then binds to a silica membrane in the column. Through centrifugation, impurities are washed away, leaving high-quality DNA ready for further analysis. It is an essential first step in many genetic studies, enabling researchers to obtain clean DNA from complex tissue matrices.
The process is efficient and user-friendly, making it suitable for various settings, from academic research to clinical diagnostics. By focusing on tissue samples, this kit addresses the unique challenges of extracting DNA from fibrous or dense materials, ensuring reliable results for downstream applications like PCR or sequencing.
Technical Specifications
| Model | Method | Sample | Format | Workflow |
|---|---|---|---|---|
| C-TIS-20R | Spin Column | Tissue | 20R | Manual |
| C-TIS-50R | Spin Column | Tissue | 50R | Manual |
| C-TIS-100R | Spin Column | Tissue | 100R | Manual |
| C-TIS-200R | Spin Column | Tissue | 200R | Manual |
| C-TIS-8R | Spin Column | Tissue | 8R | Semi-automated or fully automated |
| C-TIS-24R | Spin Column | Tissue | 24R | Semi-automated or fully automated |
| C-TIS-48R | Spin Column | Tissue | 48R | Semi-automated or fully automated |
| C-TIS-72R | Spin Column | Tissue | 72R | Semi-automated or fully automated |
| C-TIS-96R | Spin Column | Tissue | 96R | Semi-automated or fully automated |
Working Principle
The principle behind the Spin Column DNA Extraction Kit for Tissue involves a series of biochemical steps. First, the tissue sample is lysed using a buffer to break down cells and release DNA. Then, the DNA binds to a silica membrane in the spin column under specific conditions, such as in the presence of chaotropic salts.
Through centrifugation, contaminants and proteins are washed away with ethanol-based buffers, while the DNA remains bound. Finally, the pure DNA is eluted in a low-salt buffer or water, ready for use. This method leverages the affinity of DNA for silica, providing a simple yet effective way to isolate genetic material from complex tissue samples.
Performance
The performance of the Spin Column DNA Extraction Kit for Tissue is characterized by its high yield and purity of DNA. It efficiently handles a range of tissue types, including animal and human tissues, providing consistent results with minimal degradation. The kit is optimized to remove inhibitors that can affect subsequent DNA analyses, ensuring that the extracted DNA is of sufficient quality for sensitive techniques.
In practical terms, users can expect rapid processing times, often completing extractions in under an hour, which enhances workflow efficiency. The reliability of this kit makes it a trusted choice for applications requiring precise genetic data, supporting advancements in fields like medicine and biology.
Application
This kit is widely used in molecular biology and genetics research for extracting DNA from tissue samples. Common applications include genetic testing, mutation analysis, and disease research, where high-quality DNA is crucial for accurate results. It is also employed in forensic science for analyzing tissue evidence and in agricultural studies for plant tissue genotyping.
Additionally, the kit supports clinical diagnostics, such as in oncology for tumor DNA extraction, and environmental studies where tissue samples from organisms are analyzed. Its versatility makes it invaluable across multiple disciplines, facilitating discoveries and innovations in science and healthcare.
Core Features and Advantages
Core features of this kit include its high DNA recovery rate, ease of use with minimal hands-on time, and compatibility with various tissue types. It offers scalability, allowing processing of multiple samples simultaneously, which is ideal for high-throughput laboratories. The kit also includes all necessary reagents and columns, ensuring a complete solution for DNA extraction.
Advantages include reduced risk of cross-contamination due to the column-based design, and the ability to obtain PCR-ready DNA without further purification. This makes it cost-effective and time-saving, supporting rigorous scientific standards while being accessible to users with basic laboratory skills.