What is a Spin-Column DNA Extraction Kit for Plant?
A Spin-Column DNA Extraction Kit for Plant is a specialized laboratory tool designed to isolate DNA from various plant materials, such as leaves, stems, roots, seeds, or pollen. It uses a spin-column method, which involves a small column filled with a silica-based membrane that binds DNA when a sample is processed through centrifugation. This kit is essential for researchers, agriculturists, and biologists who need pure and high-quality DNA for applications like genetic analysis, plant breeding, or disease testing. The process is efficient and user-friendly, making it accessible for both beginners and experienced scientists working with plant biology.
Technical Specifications
| Model | Method | Sample | Format | Workflow |
|---|---|---|---|---|
| C-PLN-20R | Spin Column | Plant | 20R | Manual |
| C-PLN-50R | Spin Column | Plant | 50R | Manual |
| C-PLN-100R | Spin Column | Plant | 100R | Manual |
| C-PLN-200R | Spin Column | Plant | 200R | Manual |
| C-PLN-8R | Spin Column | Plant | 8R | Automation Compatible |
| C-PLN-24R | Spin Column | Plant | 24R | Automation Compatible |
| C-PLN-48R | Spin Column | Plant | 48R | Automation Compatible |
| C-PLN-72R | Spin Column | Plant | 72R | Automation Compatible |
| C-PLN-96R | Spin Column | Plant | 96R | Automation Compatible |
Working Principle
The principle behind a Spin-Column DNA Extraction Kit for Plant involves several key steps: first, plant cells are lysed using a buffer to release DNA. Then, the lysate is mixed with a binding solution that promotes DNA attachment to the silica membrane in the spin column. During centrifugation, the DNA binds to the membrane while impurities are washed away through a series of buffer steps. Finally, purified DNA is eluted in a low-salt buffer or water. This method leverages the affinity of DNA for silica under specific conditions, ensuring efficient purification without the need for toxic chemicals like phenol or chloroform.
Performance
The performance of a Spin-Column DNA Extraction Kit for Plant is highly reliable, delivering consistent results with minimal DNA degradation. It efficiently removes plant-specific contaminants, such as polysaccharides and polyphenols, which can interfere with downstream applications. With high yield and purity, the extracted DNA is suitable for sensitive techniques like PCR, sequencing, and cloning. Users can expect fast processing times, typically under an hour, and the kit is optimized for a wide range of plant species, from common crops to rare wild plants, ensuring versatility in research and agricultural settings.
Application
This kit is widely used in plant science for applications such as genetic diversity studies, molecular marker analysis, and transgenic plant detection. In agriculture, it supports crop improvement programs by enabling DNA extraction for trait identification and disease resistance testing. Environmental scientists use it to analyze plant DNA from soil or water samples for biodiversity assessments. Additionally, it is valuable in educational settings for teaching DNA extraction techniques and in forensic botany for identifying plant species in legal investigations. The kit's adaptability makes it a cornerstone tool in modern plant research and biotechnology.
Core Features and Advantages
Core features of this kit include its high efficiency in DNA binding and elution, compatibility with diverse plant tissues, and minimal hands-on time due to the spin-column design. Advantages encompass its scalability for processing multiple samples simultaneously, cost-effectiveness compared to other methods, and the production of high-integrity DNA free from inhibitors. The kit is also designed for safety, using non-hazardous reagents, and it supports automation for high-throughput applications. These features make it an ideal choice for laboratories seeking reliable and reproducible DNA extraction from plant sources.