Agricultural DNA Extraction Kit

Agricultural DNA Extraction Kit for Plant Leaves

Agricultural DNA Extraction Kit for Plant Stems

Agricultural DNA Extraction Kit for Plant Roots

Agricultural DNA Extraction Kit for Seeds

Agricultural DNA Extraction Kit for Pollen

Agricultural DNA Extraction Kit for Soil

Agricultural DNA Extraction Kit for Water

Agricultural DNA Extraction Kit for Plant Tissue

Agricultural DNA Extraction Kit for Plant Cell

Agricultural DNA Extraction Kit for FFPE Plant Samples

What is Agricultural DNA Extraction Kit?

The Agricultural DNA Extraction Kit utilizes a unique buffer formulation to efficiently remove polysaccharides, polyphenols, and other secondary metabolites found in plant samples. It is designed for extracting high-quality genomic DNA from a wide variety of agricultural research and testing samples. It is suitable for extracting a wide range of agricultural samples, including leaves, roots, stems, seeds, fruits, and petals. It also extracts from difficult-to-use samples such as succulents, starch-rich corn and rice, phenolic-rich tea and tomato, soil microbiomes, fungi, and bacteria, and processed flours, soy flours, and feeds. The kit effectively removes common inhibitors of polysaccharides, polyphenols, pigments, and secondary metabolites, providing high-yield, high-purity DNA.

The Agricultural DNA Extraction Kit simplifies the complex DNA isolation process, making it suitable for agricultural laboratories of all sizes. The extracted DNA fragments are large and have good integrity. They are not only suitable for conventional PCR, but also meet the needs of downstream molecular applications with strict requirements on DNA quality, such as PCR, restriction endonuclease digestion, genotyping, molecular identification and monitoring of plant pathogens, genetically modified organism detection (GMO), molecular marker-assisted breeding (MAS), QTL mapping, species origin identification, variety purity identification, adulteration detection, seed vigor identification involving gene bank resources, genetic diversity analysis, metagenomic analysis and next-generation sequencing (NGS).

Agricultural DNA Extraction Kit

Technical Specifications

Model Method Sample Format Workflow
AG-C-PLN Centrifugal Column Plant 20R, 50R, 100R, 200R / 8R, 24R, 48R, 72R, 96R Manual / Automation
AG-C-STM Centrifugal Column Stem 20R, 50R, 100R, 200R / 8R, 24R, 48R, 72R, 96R Manual / Automation
AG-C-ROO Centrifugal Column Root 20R, 50R, 100R, 200R / 8R, 24R, 48R, 72R, 96R Manual / Automation
AG-C-SD Centrifugal Column Seed 20R, 50R, 100R, 200R / 8R, 24R, 48R, 72R, 96R Manual / Automation
AG-C-POL Centrifugal Column Pollen 20R, 50R, 100R, 200R / 8R, 24R, 48R, 72R, 96R Manual / Automation
AG-C-SOL Centrifugal Column Soil 20R, 50R, 100R, 200R / 8R, 24R, 48R, 72R, 96R Manual / Automation
AG-M-WAT Magnetic Beads Water 20R, 50R, 100R, 200R / 8R, 24R, 48R, 72R, 96R Manual / Automation
AG-C-FFPE Centrifugal Column FFPE Tissue 20R, 50R, 100R, 200R / 8R, 24R, 48R, 72R, 96R Manual / Automation

Working Principle

The principle behind Agricultural DNA Extraction Kit involves a series of biochemical steps to isolate DNA from agricultural samples. It typically starts with cell lysis, where chemical buffers break down cell walls and membranes to release DNA into solution. This is followed by the removal of proteins, RNA, and other contaminants through enzymatic digestion or precipitation methods, ensuring that only pure DNA remains.

Next, the kit employs binding matrices, such as silica-based columns or magnetic beads, to capture the DNA while washing away impurities. The final step involves eluting the purified DNA in a buffer solution, ready for use in applications like PCR, sequencing, or genetic analysis. This principle is optimized for agricultural samples, which often contain high levels of polysaccharides or phenolics that can interfere with extraction, ensuring efficient and clean DNA recovery for accurate downstream results.

Performance

The performance of Agricultural DNA Extraction Kit is characterized by its high yield and purity of extracted DNA, essential for accurate molecular analyses. It efficiently processes a wide range of agricultural samples, from tough plant tissues like roots and stems to delicate materials such as pollen or seeds, ensuring reliable DNA recovery even from low-input samples. This consistency supports reproducible results in experiments, which is critical for agricultural research aiming to develop disease-resistant crops or improve yield.

In practical terms, the kit demonstrates robust performance under various conditions, minimizing cross-contamination and maximizing DNA integrity. It is designed to work seamlessly with common laboratory equipment, reducing processing time and increasing throughput for high-volume agricultural testing. This makes it an invaluable tool for applications requiring rapid and trustworthy DNA extraction, such as in-field diagnostics or large-scale genomic studies.

Application

Agricultural DNA Extraction Kit finds extensive applications in modern agriculture, enabling genetic studies that drive innovation in crop science. It is used for DNA extraction from plant leaves, stems, roots, seeds, and soil samples to support research on plant genetics, pathogen detection, and biodiversity assessment. For instance, it helps in identifying genetic markers for desirable traits like drought tolerance or pest resistance, facilitating the development of improved crop varieties.

Beyond plant samples, the kit is also applicable to environmental agricultural samples, such as soil or water, allowing for the study of microbial communities that influence soil health and plant growth. This supports sustainable farming practices by enabling monitoring of genetically modified organisms (GMOs) or detecting plant diseases early. In educational settings, it serves as a hands-on tool for teaching molecular biology concepts related to agriculture, making complex processes accessible to students and researchers alike.

Core Features and Advantages

The core features of Agricultural DNA Extraction Kit include its versatility in handling diverse agricultural samples, high DNA yield and purity, and user-friendly protocol that minimizes hands-on time. It is designed to be compatible with a wide range of plant and environmental materials, from fibrous tissues to microbial-rich soils, offering flexibility for various research needs. The kit often includes optimized buffers and reagents that prevent DNA degradation, enhancing the reliability of extracted genetic material.

Key advantages include its cost-effectiveness, scalability for high-throughput applications, and reduced risk of contamination compared to traditional methods. It supports rapid processing, making it ideal for time-sensitive agricultural studies, such as disease outbreak investigations or field-based research. Additionally, the kit is often validated for use with downstream molecular techniques, ensuring seamless integration into agricultural genomics workflows and contributing to advancements in food security and sustainable agriculture.

Application
Agriculture
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