What is Research DNA Extraction Kit for Saliva?
A Research DNA Extraction Kit for Saliva is a specialized laboratory tool designed to isolate high-quality DNA from saliva samples, which are commonly used in scientific studies due to their non-invasive collection. Saliva contains cells from the oral cavity, and this kit efficiently extracts genetic material for various research applications, ensuring purity and yield suitable for downstream analyses like PCR, sequencing, or genotyping. It simplifies the process, making it accessible for researchers in fields such as genetics, epidemiology, and biomedical sciences.
Technical Specifications
| Model | Method | Sample | Application | Format | Workflow |
|---|---|---|---|---|---|
| R-C-SAL-20R | Column | Saliva | Research | 20R | Manual |
| R-C-SAL-50R | Column | Saliva | Research | 50R | Manual |
| R-C-SAL-100R | Column | Saliva | Research | 100R | Manual |
| R-C-SAL-200R | Column | Saliva | Research | 200R | Manual |
| R-C-SAL-8R | Column | Saliva | Research | 8R | Semi-automated or Full Automation |
| R-C-SAL-24R | Column | Saliva | Research | 24R | Semi-automated or Full Automation |
| R-C-SAL-48R | Column | Saliva | Research | 48R | Semi-automated or Full Automation |
| R-C-SAL-72R | Column | Saliva | Research | 72R | Semi-automated or Full Automation |
| R-C-SAL-96R | Column | Saliva | Research | 96R | Semi-automated or Full Automation |
Working Principle
The principle behind this kit involves lysing the cells in saliva to release DNA, followed by binding the DNA to a solid phase, such as silica membranes or magnetic beads, and then washing away impurities. Finally, the pure DNA is eluted in a buffer solution. This method leverages chemical and physical processes to ensure efficient separation from proteins, salts, and other contaminants, resulting in clean DNA ready for research applications.
Performance
The performance of this kit is optimized for saliva samples, delivering consistent and reliable DNA extraction with minimal contamination. It achieves high DNA yield and purity, as measured by absorbance ratios, ensuring that the extracted DNA is ideal for sensitive research techniques. Benchmarks show that it outperforms traditional methods in terms of speed and efficiency, reducing processing time while maintaining integrity for long-term storage or immediate use in experiments.
Application
This kit is widely used in research settings for applications such as genetic studies, population genetics, and disease research, where saliva serves as a convenient source of DNA. It supports projects involving biomarker discovery, pharmacogenomics, and microbiome analysis, enabling scientists to explore genetic variations and associations without invasive sampling. Its versatility makes it a go-to choice for academic laboratories and research institutions focusing on human health and biological sciences.
Core Features and Advantages
Key features include a user-friendly protocol that requires minimal hands-on time, high scalability for processing multiple samples, and compatibility with automated systems. Advantages encompass its ability to handle varying sample volumes, provide reproducible results, and reduce the risk of cross-contamination, making it a cost-effective and reliable solution for high-throughput research environments.