What is Animal DNA Extraction Kit for Hair?
The Animal DNA Extraction Kit for Hair is a specialized laboratory tool designed to isolate DNA from hair samples of animals. Hair is a common and non-invasive source of genetic material, making it ideal for studies in wildlife conservation, veterinary diagnostics, and genetic research. This kit simplifies the process by providing all necessary reagents and components to efficiently extract high-quality DNA, which can then be used for various downstream applications like PCR, sequencing, or genotyping.
Using this kit, researchers can obtain DNA from even small or degraded hair samples, which is crucial when working with wild animals or historical specimens. It ensures that the extracted DNA is pure and free from contaminants, allowing for accurate and reliable results in genetic analyses. This makes it an essential tool for anyone involved in animal genetics or biodiversity studies.
Technical Specifications
| Model | Method | Sample | Application | Format | Workflow |
|---|---|---|---|---|---|
| A-C-HAI-20R | Column | Hair | Animal | 20R | Manual |
| A-C-HAI-50R | Column | Hair | Animal | 50R | Manual |
| A-C-HAI-100R | Column | Hair | Animal | 100R | Manual |
| A-C-HAI-200R | Column | Hair | Animal | 200R | Manual |
| A-C-HAI-8R | Column | Hair | Animal | 8R | Semi-automated or Full Automation |
| A-C-HAI-24R | Column | Hair | Animal | 24R | Semi-automated or Full Automation |
| A-C-HAI-48R | Column | Hair | Animal | 48R | Semi-automated or Full Automation |
| A-C-HAI-72R | Column | Hair | Animal | 72R | Semi-automated or Full Automation |
| A-C-HAI-96R | Column | Hair | Animal | 96R | Semi-automated or Full Automation |
Working Principle
The principle behind the Animal DNA Extraction Kit for Hair involves a combination of mechanical and chemical steps to break down the hair structure and release DNA. Hair samples are first treated with a lysis buffer that disrupts the cellular and protein components, including the tough keratin outer layer. This is followed by the addition of enzymes or other reagents that digest proteins and other impurities, leaving the DNA intact.
Next, the kit uses a purification method, such as spin-column technology or magnetic beads, to bind the DNA and separate it from cellular debris. Washing steps remove any remaining contaminants, and finally, the DNA is eluted in a buffer ready for use. This process ensures high recovery rates and minimizes degradation, making it efficient for various animal hair types.
Performance
The performance of the Animal DNA Extraction Kit for Hair is optimized to handle the unique challenges of hair samples, such as the presence of keratin and other proteins that can inhibit DNA extraction. It delivers consistent yields of high-molecular-weight DNA with excellent purity, as measured by absorbance ratios. This means that the DNA is suitable for sensitive techniques like next-generation sequencing, where quality is paramount for successful outcomes.
In tests, this kit has shown high efficiency in extracting DNA from a variety of animal hairs, including those from mammals, birds, and other species. It minimizes the risk of cross-contamination and ensures reproducibility across multiple samples, which is vital for large-scale studies or forensic applications. Users can expect reliable performance even with limited starting material, making it a cost-effective choice for labs.
Application
The Animal DNA Extraction Kit for Hair finds applications in numerous fields, including wildlife biology, where it is used to study population genetics, migration patterns, and species identification from hair collected in the field. In veterinary medicine, it aids in diagnosing genetic disorders or infections by analyzing DNA from pet or livestock hair. Additionally, it is valuable in forensic science for identifying animals involved in crimes or accidents, as well as in conservation efforts to monitor endangered species without harming them.
Beyond these, the kit supports research in evolutionary biology, allowing scientists to extract DNA from historical or museum specimens to understand genetic changes over time. It is also used in agricultural settings to improve breeding programs by analyzing genetic traits from animal hair, ensuring better herd management and productivity.
Core Features and Advantages
The core features of this kit include its user-friendly protocol that requires minimal hands-on time, reducing the risk of errors and making it accessible even for beginners. It is designed to work with small amounts of hair, which is beneficial when samples are scarce. The kit also offers high scalability, allowing processing of single samples or high-throughput batches, and it is compatible with automated systems for increased efficiency.
Advantages include its ability to handle challenging samples, such as old or contaminated hair, and produce DNA that is free from inhibitors commonly found in hair. This leads to more accurate genetic analyses and reduces the need for repeat experiments. Additionally, the kit is cost-effective and comes with all necessary components, saving time and resources in the lab.