What is Research DNA Extraction Kit for Tissue?
A Research DNA Extraction Kit for Tissue is a specialized laboratory tool designed for scientists and researchers who need to isolate high-quality DNA from tissue samples. These kits provide all the necessary reagents and materials to efficiently break down tissue structures, release DNA, and purify it for downstream applications. Tissue samples, whether from animals, plants, or humans, often contain complex matrices that can interfere with DNA extraction, making these kits essential for obtaining clean and intact genetic material.
Using such a kit simplifies the extraction process, ensuring consistency and reliability in research outcomes. It is commonly employed in molecular biology, genetics, and biomedical studies, where accurate DNA analysis is crucial. The kit handles various tissue types, including fresh, frozen, or preserved samples, adapting to different research needs without compromising on yield or quality.
Technical Specifications
| Model | Method | Sample | Application | Format | Workflow |
|---|---|---|---|---|---|
| R-C-TIS-20R | Column | Tissue | Research | 20R | Manual |
| R-C-TIS-50R | Column | Tissue | Research | 50R | Manual |
| R-C-TIS-100R | Column | Tissue | Research | 100R | Manual |
| R-C-TIS-200R | Column | Tissue | Research | 200R | Manual |
| R-C-TIS-8R | Column | Tissue | Research | 8R | Semi-automated or Full Automation |
| R-C-TIS-24R | Column | Tissue | Research | 24R | Semi-automated or Full Automation |
| R-C-TIS-48R | Column | Tissue | Research | 48R | Semi-automated or Full Automation |
| R-C-TIS-72R | Column | Tissue | Research | 72R | Semi-automated or Full Automation |
| R-C-TIS-96R | Column | Tissue | Research | 96R | Semi-automated or Full Automation |
Working Principle
The principle behind a Research DNA Extraction Kit for Tissue involves a series of biochemical steps to isolate DNA. First, the tissue is lysed using buffers that break down cell membranes and nuclear envelopes, releasing DNA into solution. Protease enzymes may be added to digest proteins that could co-purify with DNA.
Next, the DNA is separated from other cellular components through binding to a solid phase, such as silica columns or magnetic beads, in the presence of chaotropic salts. Washing steps remove impurities like proteins, RNA, and salts, leaving pure DNA. Finally, the DNA is eluted in a buffer that preserves its integrity for subsequent research applications.
Performance
The performance of a Research DNA Extraction Kit for Tissue is measured by its ability to deliver high DNA yield and purity from diverse tissue sources. These kits are optimized to minimize degradation and contamination, resulting in DNA that is suitable for sensitive techniques like PCR, sequencing, and cloning. Efficiency is key, with many kits completing extraction in under an hour, saving valuable time in busy research settings.
Consistency across multiple samples is another critical aspect, allowing researchers to compare results accurately. Performance testing often shows high success rates even with challenging tissues, such as those with high lipid content or degradation, ensuring that the extracted DNA meets the stringent requirements of modern genetic research.
Application
Research DNA Extraction Kits for Tissue are widely used in academic, clinical, and industrial research environments. They support studies in genomics, where DNA from tissues is analyzed to understand gene expression, mutations, and disease mechanisms. In cancer research, for instance, these kits help extract DNA from tumor tissues to identify genetic alterations.
Applications extend to evolutionary biology, where tissue samples from various species are processed to study phylogenetic relationships. Additionally, these kits are invaluable in biotechnology for developing new therapies and in agricultural research for improving crop traits through genetic analysis.
Core Features and Advantages
Core features of these kits include user-friendly protocols that require minimal hands-on time, making them accessible even to those new to DNA extraction. They often come with pre-measured reagents to reduce errors and ensure reproducibility. High scalability allows processing of single samples or high-throughput batches, catering to diverse research scales.
Advantages include high DNA purity, which is essential for sensitive downstream analyses, and compatibility with automated systems for increased efficiency. The kits are designed to be cost-effective, reducing the need for additional equipment or reagents, and they support a wide range of tissue types, enhancing their versatility in research settings.