What is Clinical DNA Extraction Kit for Mutation Analysis?
The Clinical DNA Extraction Kit for Mutation Analysis is a specialized laboratory tool designed to isolate high-purity DNA from clinical samples for detecting genetic mutations. This kit is essential in medical diagnostics, as it ensures the DNA extracted is free from contaminants that could interfere with accurate mutation detection. By providing clean DNA, it supports downstream applications like PCR and sequencing, which are critical for identifying disease-related genetic changes.
This kit is optimized for use with various clinical specimens, including blood, tissue, and saliva, making it versatile for different diagnostic needs. Its design focuses on efficiency and reliability, ensuring that healthcare professionals can obtain consistent results. The process involves breaking down cells to release DNA, followed by purification steps to remove proteins and other impurities, resulting in DNA ready for mutation analysis.
Technical Specifications
| Model | Method | Sample | Application | Format | Workflow |
|---|---|---|---|---|---|
| C-M-BLD-20R | Magnetic Beads | Blood | Clinical | 20R | Manual |
| C-M-BLD-50R | Magnetic Beads | Blood | Clinical | 50R | Manual |
| C-M-BLD-100R | Magnetic Beads | Blood | Clinical | 100R | Manual |
| C-M-BLD-200R | Magnetic Beads | Blood | Clinical | 200R | Manual |
| C-M-BLD-8R | Magnetic Beads | Blood | Clinical | 8R | Semi-automated or Full Automation |
| C-M-BLD-24R | Magnetic Beads | Blood | Clinical | 24R | Semi-automated or Full Automation |
| C-M-BLD-48R | Magnetic Beads | Blood | Clinical | 48R | Semi-automated or Full Automation |
| C-M-BLD-72R | Magnetic Beads | Blood | Clinical | 72R | Semi-automated or Full Automation |
| C-M-BLD-96R | Magnetic Beads | Blood | Clinical | 96R | Semi-automated or Full Automation |
Working Principle
The principle behind the Clinical DNA Extraction Kit for Mutation Analysis involves lysing cells to release DNA, followed by binding the DNA to a solid phase, such as silica-based membranes or magnetic beads, and then washing away impurities. The purified DNA is eluted in a buffer that preserves its integrity for subsequent analysis. This method leverages chemical and physical processes to ensure efficient separation of DNA from other cellular components.
Key steps include the use of lysis buffers to break down cell membranes and nucleases to degrade proteins, ensuring that the DNA remains intact. The binding step exploits the affinity of DNA for specific surfaces under optimal conditions, while wash steps remove contaminants. This principled approach guarantees that the extracted DNA is of high quality and suitable for sensitive mutation detection techniques.
Performance
The Clinical DNA Extraction Kit for Mutation Analysis delivers exceptional performance by yielding high-quality DNA with minimal fragmentation, which is crucial for accurate mutation detection. It achieves high recovery rates, meaning more DNA is extracted from each sample, reducing the need for large sample volumes. This is particularly important in clinical settings where sample availability may be limited.
Tests show that the kit consistently produces DNA with high purity, as measured by absorbance ratios, ensuring that downstream analyses are not compromised by contaminants. Its performance is validated across a range of clinical samples, providing reliable results for various diagnostic applications. This reliability helps laboratories maintain high standards in genetic testing and research.
Application
This kit is primarily used in clinical laboratories for extracting DNA from patient samples to analyze genetic mutations associated with diseases such as cancer, inherited disorders, and infectious conditions. It supports applications like next-generation sequencing, PCR-based assays, and microarray analysis, which are fundamental in personalized medicine and diagnostic workflows.
Beyond mutation analysis, it can be applied in research studies focusing on genetic variations and their clinical implications. Its versatility makes it suitable for use in hospitals, diagnostic centers, and research institutions, aiding in the early detection and monitoring of genetic diseases. This broad applicability underscores its importance in advancing medical science and improving patient outcomes.
Core Features and Advantages
The core features of this kit include its high efficiency in DNA yield and purity, user-friendly protocol that minimizes hands-on time, and compatibility with automated systems for high-throughput processing. These features make it ideal for busy clinical environments where speed and accuracy are paramount. Additionally, the kit is designed to reduce cross-contamination risks, ensuring reliable results in mutation analysis.
Advantages encompass its scalability, allowing it to be used for both small-scale and large-scale extractions, and its cost-effectiveness due to reduced reagent consumption. The kit also offers stability and long shelf life, making it a dependable choice for laboratories. These benefits collectively enhance diagnostic workflows, supporting timely and accurate genetic testing.