What is Animal DNA Extraction Kit for Fur?
An Animal DNA Extraction Kit for Fur is a specialized scientific toolkit designed to isolate high-quality DNA from animal fur samples. Fur contains hair follicles and skin cells that harbor genetic material, making it a valuable non-invasive source for DNA analysis. These kits provide all necessary reagents and components to efficiently break down the keratinized structure of fur and release intact DNA molecules. The extracted DNA can then be used for various downstream applications such as genetic studies, species identification, or forensic investigations.
Using such a kit eliminates the need for complex laboratory procedures and allows researchers, veterinarians, or conservationists to obtain reliable genetic data from minimal fur samples. The process is optimized to handle challenges like environmental degradation or contaminants often present in fur collected from wildlife or domestic animals. This makes the kit an essential tool in fields like zoology, ecology, and veterinary science where non-destructive sampling is preferred.
Technical Specifications
| Model | Method | Sample | Application | Format | Workflow |
|---|---|---|---|---|---|
| A-C-FEA-20R | Column | Feather | Animal | 20R | Manual |
| A-C-FEA-50R | Column | Feather | Animal | 50R | Manual |
| A-C-FEA-100R | Column | Feather | Animal | 100R | Manual |
| A-C-FEA-200R | Column | Feather | Animal | 200R | Manual |
| A-C-FEA-8R | Column | Feather | Animal | 8R | Semi-automated or Full Automation |
| A-C-FEA-24R | Column | Feather | Animal | 24R | Semi-automated or Full Automation |
| A-C-FEA-48R | Column | Feather | Animal | 48R | Semi-automated or Full Automation |
| A-C-FEA-72R | Column | Feather | Animal | 72R | Semi-automated or Full Automation |
| A-C-FEA-96R | Column | Feather | Animal | 96R | Semi-automated or Full Automation |
Working Principle
The principle behind the Animal DNA Extraction Kit for Fur involves a series of biochemical steps to lyse cells and purify DNA. First, the fur sample is treated with a lysis buffer that breaks down the tough keratin and cellular structures, releasing DNA into solution. Proteolytic enzymes may be included to digest proteins and other contaminants, ensuring that the DNA is not degraded. Next, binding conditions are optimized to capture DNA onto a solid phase, such as silica columns or magnetic beads, which selectively isolate DNA from impurities.
Washing steps remove residual inhibitors like salts, dyes, or organic matter, followed by elution in a buffer that yields pure, concentrated DNA. This principle leverages the affinity of DNA for specific surfaces under controlled pH and salt conditions, making the process efficient and scalable. The kit's design ensures that even trace amounts of DNA from shed fur can be successfully extracted, making it ideal for low-input samples common in wildlife studies.
Performance
The Animal DNA Extraction Kit for Fur delivers consistent and high-performance results by ensuring maximum DNA yield and purity from even small or aged fur samples. It effectively removes inhibitors such as pigments, oils, and external contaminants that are common in fur, resulting in DNA that is suitable for sensitive techniques like PCR, sequencing, or genotyping. Performance tests show that the kit maintains DNA integrity, with minimal fragmentation, which is crucial for applications requiring long-range amplification or detailed genetic analysis.
Users can expect rapid processing times, typically completing extractions within a few hours, depending on the sample quantity and protocol. The kit's robustness allows it to perform well across a wide range of animal species, from mammals with dense fur to birds with feathers, adapting to variations in sample quality. This reliability makes it a trusted choice for both field research and laboratory settings, ensuring reproducible outcomes every time.
Application
The Animal DNA Extraction Kit for Fur is widely used in diverse applications, including wildlife conservation, where it helps in monitoring endangered species through non-invasive genetic sampling. Researchers extract DNA from fur collected via hair traps or brushes, enabling population studies, kinship analysis, and biodiversity assessments without harming animals. In veterinary medicine, the kit aids in diagnosing genetic disorders, identifying breeds, or tracking hereditary traits in pets and livestock based on fur samples.
Additionally, it finds applications in forensic science for identifying animals involved in crimes or accidents, such as hit-and-run cases where fur evidence is collected. In academic research, the kit supports studies on evolution, adaptation, and genetics across animal taxa. Its versatility also extends to zoos, museums, and environmental agencies, facilitating DNA banking and genetic diversity projects from fur archives or field collections.
Core Features and Advantages
The core features of the Animal DNA Extraction Kit for Fur include its high sensitivity, allowing extraction from minimal fur samples, and its compatibility with a broad range of animal species. It offers user-friendly protocols that require no specialized equipment, making it accessible for both novice and experienced users. The kit is designed to be rapid, reducing processing time compared to traditional methods, and it includes all necessary components pre-measured for convenience, minimizing the risk of errors.
Advantages include high DNA yield and purity, which are critical for downstream applications like PCR and sequencing, ensuring reliable results. The kit is also cost-effective, reducing the need for multiple reagents or external services. Its non-invasive nature supports ethical research practices by allowing DNA collection without harming animals. Furthermore, it is scalable for high-throughput workflows, enabling processing of multiple samples simultaneously in research or diagnostic labs.