What is Forensic DNA Extraction Kit for FFPE Samples?
Forensic DNA Extraction Kit for FFPE samples is a specialized tool designed to isolate DNA from formalin-fixed paraffin-embedded (FFPE) tissues, which are commonly preserved for long-term storage in forensic and medical investigations. FFPE samples are challenging because the fixation process can damage DNA, making extraction difficult. This kit uses optimized buffers and methods to break down the paraffin and reverse the effects of formalin, allowing for the recovery of high-quality DNA that can be used in downstream forensic analyses.
In forensic science, FFPE samples are often critical evidence from cold cases or historical investigations where tissues were preserved for future examination. This kit ensures that even degraded or cross-linked DNA can be extracted efficiently, providing reliable results for identification, paternity testing, or criminal investigations. It is an essential resource for forensic laboratories dealing with archived biological materials.
Technical Specifications
| Model | Method | Sample | Application | Format | Workflow |
|---|---|---|---|---|---|
| F-M-FFPE-20R | Magnetic Beads | FFPE Tissue | Forensic | 20R | Manual |
| F-M-FFPE-50R | Magnetic Beads | FFPE Tissue | Forensic | 50R | Manual |
| F-M-FFPE-100R | Magnetic Beads | FFPE Tissue | Forensic | 100R | Manual |
| F-M-FFPE-200R | Magnetic Beads | FFPE Tissue | Forensic | 200R | Manual |
| F-M-FFPE-8R | Magnetic Beads | FFPE Tissue | Forensic | 8R | Semi-automated or Full Automation |
| F-M-FFPE-24R | Magnetic Beads | FFPE Tissue | Forensic | 24R | Semi-automated or Full Automation |
| F-M-FFPE-48R | Magnetic Beads | FFPE Tissue | Forensic | 48R | Semi-automated or Full Automation |
| F-M-FFPE-72R | Magnetic Beads | FFPE Tissue | Forensic | 72R | Semi-automated or Full Automation |
| F-M-FFPE-96R | Magnetic Beads | FFPE Tissue | Forensic | 96R | Semi-automated or Full Automation |
Working Principle
The principle behind the Forensic DNA Extraction Kit for FFPE samples involves deparaffinization to remove the paraffin wax, followed by digestion to break down proteins and reverse formalin-induced cross-links that damage DNA. Specialized lysis buffers are used to solubilize the tissue and release DNA, while purification steps remove inhibitors like formalin residues and other contaminants. This ensures that the extracted DNA is clean and amplifiable.
By leveraging enzymatic and chemical processes, the kit efficiently isolates DNA while preserving its integrity. The method is optimized for forensic needs, focusing on maximizing yield from limited or degraded samples, which is crucial for obtaining reliable results in DNA-based investigations where sample quality is often compromised.
Performance
The Forensic DNA Extraction Kit for FFPE samples delivers high performance by achieving consistent DNA yields with purity suitable for sensitive forensic applications like PCR and sequencing. It is designed to handle the tough nature of FFPE tissues, often subjected to years of storage, by minimizing DNA fragmentation and inhibitor carryover. Performance tests show that it can extract DNA from small or degraded samples, making it ideal for challenging forensic cases where evidence is limited.
This kit's performance is validated through rigorous quality controls, ensuring that extracted DNA meets the standards required for forensic DNA profiling and other analytical techniques. It reduces processing time compared to traditional methods, offering a reliable solution for laboratories aiming to maximize efficiency without compromising on accuracy in DNA recovery from FFPE specimens.
Application
The Forensic DNA Extraction Kit for FFPE samples is primarily used in forensic investigations to extract DNA from archived tissue samples, such as those from autopsies or cold cases. It enables DNA analysis for identifying individuals, solving crimes, or confirming biological relationships in legal contexts. Applications extend to research in historical forensics, where preserved samples need to be analyzed for genetic information.
Beyond forensics, this kit can be applied in medical-legal cases, anthropological studies, and disaster victim identification, where FFPE samples are common. It supports various downstream techniques, including STR analysis and next-generation sequencing, making it versatile for comprehensive forensic science workflows that rely on high-integrity DNA from challenging sources.
Core Features and Advantages
Core features of this kit include optimized reagents for deparaffinization and DNA recovery, high compatibility with various FFPE sample types, and a user-friendly protocol that reduces hands-on time. It offers advantages such as high DNA yield and purity, minimal inhibitor carryover, and reliability for forensic applications. The kit is designed to handle small or difficult samples, making it a robust choice for challenging investigations.
Additional advantages include scalability for processing multiple samples simultaneously and compatibility with automated systems, enhancing throughput in busy forensic labs. Its effectiveness in dealing with aged or cross-linked DNA provides a significant edge over conventional extraction methods, ensuring that critical evidence can be analyzed with confidence.