What is Forensic DNA Extraction Kit for Tissue?
Forensic DNA Extraction Kit for Tissue is a specialized chemical set used in the collection and processing phases of DNA analysis. This kit plays a crucial role in forensic investigations, enabling the accurate extraction of DNA from bodily tissue samples. These tissues can be recovered from various sources like injuries, surgical procedures, or post-mortem examinations, and are often used in criminal cases, missing person identifications, or paternity disputes. By isolating DNA in a pure, uncontaminated form, it supports reliable results that withstand legal scrutiny.
The kit is designed to handle the unique challenges of tissue samples, which may be degraded, contaminated, or present in small quantities. It ensures that even old or damaged tissues yield usable DNA for analysis. This process is foundational in forensic science, turning biological evidence into actionable data that helps solve crimes and provide justice.
Technical Specifications
| Model | Method | Sample | Application | Format | Workflow |
|---|---|---|---|---|---|
| F-C-TIS-20R | Column | Tissue | Forensic | 20R | Manual |
| F-C-TIS-50R | Column | Tissue | Forensic | 50R | Manual |
| F-C-TIS-100R | Column | Tissue | Forensic | 100R | Manual |
| F-C-TIS-200R | Column | Tissue | Forensic | 200R | Manual |
| F-C-TIS-8R | Column | Tissue | Forensic | 8R | Semi-automated or Full Automation |
| F-C-TIS-24R | Column | Tissue | Forensic | 24R | Semi-automated or Full Automation |
| F-C-TIS-48R | Column | Tissue | Forensic | 48R | Semi-automated or Full Automation |
| F-C-TIS-72R | Column | Tissue | Forensic | 72R | Semi-automated or Full Automation |
| F-C-TIS-96R | Column | Tissue | Forensic | 96R | Semi-automated or Full Automation |
Working Principle
The principle behind this kit involves a series of biochemical steps to break down tissue cells and release DNA. It typically uses lysis buffers to dissolve cell membranes and proteins, followed by purification methods to separate DNA from other cellular components. This ensures that the extracted DNA is clean and ready for further analysis.
Specific to tissue samples, the kit may include enzymes or mechanical processes to handle tough fibrous materials, ensuring efficient DNA recovery. The process is designed to be gentle yet effective, preserving DNA integrity for accurate forensic testing.
Performance
The performance of this DNA extraction kit is characterized by high efficiency and consistency in recovering DNA from tissue specimens. It is optimized to work with a wide range of tissue types, including fresh, frozen, or fixed tissues, delivering high yields of pure DNA with minimal degradation. This ensures that downstream applications, such as PCR amplification or sequencing, are successful and produce accurate results.
In forensic contexts, the kit's reliability is paramount, as it must perform under varying conditions and with samples that may have been exposed to environmental factors. Rigorous testing shows that it maintains high performance, reducing the risk of false negatives or contaminants. This makes it a trusted tool in forensic laboratories worldwide.
Application
This kit is primarily applied in forensic science for extracting DNA from tissue samples to aid in criminal investigations, disaster victim identification, and legal cases. It is used to analyze tissues from crime scenes, such as skin fragments, organ tissues, or biopsy samples, to identify individuals or establish biological relationships.
Beyond forensics, it finds use in research settings for studying genetic markers in tissue samples, as well as in medical and anthropological studies. Its versatility allows it to support various DNA-based analyses, contributing to advancements in science and law enforcement.
Core Features and Advantages
Core features of this kit include high specificity for tissue samples, user-friendly protocols that minimize handling errors, and compatibility with automated systems for high-throughput processing. It offers advantages such as rapid extraction times, high DNA yield, and low risk of cross-contamination, which are critical in forensic applications.
Additionally, the kit is designed to be robust, performing well with degraded or challenging samples. Its reliability and ease of use make it a preferred choice for forensic experts, enhancing the efficiency and accuracy of DNA analysis in legal contexts.