What is Animal DNA Extraction Kit for Bone?
The Animal DNA Extraction Kit for Bone is a specialized laboratory tool designed to isolate and purify DNA from bone samples derived from animals. This kit is essential for researchers, veterinarians, and conservationists who need to study genetic material from skeletal remains for various purposes such as species identification, evolutionary studies, or disease diagnosis. Bones are challenging samples due to their hardness and low DNA content, but this kit streamlines the extraction process efficiently.
It provides all necessary components in a single package, ensuring that users can obtain high-quality DNA without contamination. The kit is user-friendly and suitable for both novice and experienced laboratory personnel, making genetic analysis more accessible and reliable in fields like wildlife biology and forensic science.
Technical Specifications
| Model | Method | Sample | Application | Format | Workflow |
|---|---|---|---|---|---|
| A-C-BM-20R | Column | Bone Marrow | Animal | 20R | Manual |
| A-C-BM-50R | Column | Bone Marrow | Animal | 50R | Manual |
| A-C-BM-100R | Column | Bone Marrow | Animal | 100R | Manual |
| A-C-BM-200R | Column | Bone Marrow | Animal | 200R | Manual |
| A-C-BM-8R | Column | Bone Marrow | Animal | 8R | Semi-automated or Full Automation |
| A-C-BM-24R | Column | Bone Marrow | Animal | 24R | Semi-automated or Full Automation |
| A-C-BM-48R | Column | Bone Marrow | Animal | 48R | Semi-automated or Full Automation |
| A-C-BM-72R | Column | Bone Marrow | Animal | 72R | Semi-automated or Full Automation |
| A-C-BM-96R | Column | Bone Marrow | Animal | 96R | Semi-automated or Full Automation |
Working Principle
The principle behind the Animal DNA Extraction Kit for Bone involves a series of biochemical steps to break down the hard bone matrix and release DNA. First, the bone sample is ground or digested to disrupt its structure. Then, lysis buffers are used to dissolve cellular components and proteins, freeing the DNA. Specialized reagents help in precipitating and purifying the DNA, removing impurities like minerals and inhibitors that are common in bone tissue.
This process ensures that the DNA is isolated in a form that is ready for further analysis. The kit leverages silica-based or magnetic bead technology to bind DNA, facilitating easy separation and elution, which enhances yield and purity for reliable genetic studies.
Performance
The performance of the Animal DNA Extraction Kit for Bone is optimized to handle the unique challenges posed by bone tissue, such as degradation and mineralization. It consistently yields DNA with high purity and integrity, which is crucial for downstream applications like PCR amplification and sequencing. Users can expect efficient recovery rates, even from old or fossilized bones, ensuring that valuable genetic information is not lost.
In comparative studies, this kit often outperforms generic extraction methods by reducing processing time and minimizing sample loss. Its reliability makes it a preferred choice for critical research projects where accurate DNA data is paramount, supporting advancements in animal genetics and biodiversity conservation.
Application
This kit finds extensive application in animal genetics research, allowing scientists to extract DNA from bone samples for studies on population genetics, phylogenetics, and hereditary diseases. It is widely used in veterinary diagnostics to identify genetic disorders in animals based on bone marrow or skeletal DNA. Additionally, in forensics, it aids in wildlife crime investigations by helping to trace and identify species from bone remnants.
Environmental and conservation biologists use it to monitor endangered species through DNA analysis of bone samples collected from natural habitats. The kit also supports archaeological research, enabling the extraction of ancient DNA from animal bones to study evolutionary history and domestication processes.
Core Features and Advantages
The core features of this kit include high efficiency in DNA extraction from tough bone samples, user-friendly protocols that require minimal technical expertise, and compatibility with various downstream molecular techniques. It offers advantages such as reduced risk of contamination, fast processing times, and scalability for high-throughput applications.
Compared to traditional methods, it provides consistent results with less sample input, making it cost-effective and ideal for laboratories with limited resources. The kit's design ensures that it can handle a wide range of bone types, from fresh to ancient specimens, offering versatility and reliability in diverse research settings.